L-ERGOTHIONEINE 99% BY HPLC
Premium Antioxidant for Nutraceutical & Cosmeceutical Applications
1. Product Overview
L-Ergothioneine (ERT) is a naturally occurring sulfur-containing amino acid derived from microbial fermentation, primarily found in mushrooms such as Tricholoma matsutake and Hericium erinaceus . Our product is a high-purity (≥99% by HPLC) crystalline powder with CAS No. 497-30-3, validated through rigorous chromatographic analysis . It exhibits exceptional stability in pH 4.0-6.0 formulations, making it ideal for skincare serums, lotions, and oral supplements .
Key Features:
- Purity: ≥99% (HPLC-certified, batch-specific COA provided)
- Molecular Formula: C₉H₁₅N₃O₂S | Molecular Weight: 229.3 g/mol
- Solubility: 125 mg/mL in water (ultrasonic-assisted)
- Certifications: Non-GMO, Allergen-Free, Vegan-Friendly
2. Quality Control & HPLC Methodology
Our analytical process aligns with peer-reviewed protocols :
- Sample Preparation: 1 mL culture medium boiled at 94°C for 10 min, vortexed (1600 rpm/30 min), centrifuged (10,000×g/5 min), and stored at -20°C if needed .
- HPLC System: Dionex Ultimate 3000 with Chromeleon software .
- Column: Cortecs UPLC T3 (2.1×150 mm, 1.6 μm particles, 120 Å pore size) .
- Mobile Phase: Gradient elution from 0.1% formic acid to 70% acetonitrile/0.1% formic acid, flow rate 0.3 mL/min .
- Detection: UV absorbance at 254 nm, LOQ 0.15 mmol/L .
Validation Metrics:
- Linearity: 0.3–10 mmol/L (R² >0.99)
- Precision: ≤6% RSD (intra-/inter-assay)
- Recovery: ~100% accuracy
3. Health Benefits & Applications
3.1 Nutraceutical Uses
- Mitochondrial Protection: Reduces oxidative stress by scavenging hydroxyl radicals (IC₅₀: 2.5 μM) and superoxide anions .
- Telomere Support: Delays age-related telomere shortening in vitro .
- Dosage: Clinical studies suggest 5–20 mg/day elevates plasma ERT levels significantly .
Recommended Products:
- Capsules: 100 μg–5 mg per serving (e.g., LifeExtension’s Essential Youth) .
- Synergistic Blends: Combines with ferulic acid for enhanced bioavailability .
3.2 Cosmeceutical Uses
- Anti-Aging: Inhibits tyrosinase (40% reduction at 0.5% concentration) and protects elastin from UV degradation .
- Formulation Guidelines:
- pH: 4.0–6.0 (optimal stability)
- Concentration: 0.5–2.0% in serums/lotions
- Compatibility: Stable with vitamin C, hyaluronic acid, and peptides .
4. Technical Specifications
| Parameter | Specification | Reference |
|---|---|---|
| Appearance | White crystalline powder | |
| Purity (HPLC) | ≥99% | |
| Melting Point | 275–280°C (decomposes) | |
| Storage | 2–8°C in airtight container | |
| Shelf Life | 24 months |
5. Safety & Regulatory Compliance
- Safety Data: LD₅₀ >2000 mg/kg (oral, rats); no adverse effects in human trials .
- Certifications: EU Novel Food Approval (Ergoneine®), FDA GRAS .
- Handling: Use NIOSH-approved respirators and chemical-resistant gloves during industrial processing .
6. Global Supply & Packaging
- Packaging Options: 1 kg, 10 kg, 1000 kg (aluminum foil bags with desiccant) .
- Lead Time: 3–5 business days (global shipping via DHL/FedEx) .
- Markets Served: USA, EU, Australia, Japan, Brazil .
7. Key Words
- Keywords: “L-Ergothioneine 99% HPLC”, “Natural Antioxidant Supplier”, “Ergothioneine for Skincare”.
- Include CAS 497-30-3, purity claims, and application terms.
