igoa ole oloa:β-NADPH
Isi igoa:β-NADPH|beta-Nicotinamide adenine dinucleotide 2′-phosphate fa'aititia le tetrasodium salt hydrate
Synonym:beta-NADPH; 2′-NADPH hydrate; Coenzyme II fa'aitiitia le masima tetrasodium; Dihydronicotinamide adenine dinucleotide phosphate tetrasodium masima; NADPH Na4; TPNH2 Na4; Triphosphopyridine nucleotide faʻaitiitia le masima tetrasodium
Numera CAS:2646-71-1
Numera EINECS:220-163-3
Mama: ≥98%
Teuga vevela: -20°C
Fa'aaliga: Pa'epa'e e pa'epa'e samasama
Faʻamatalaga o oloa: β-NADPH (β-Nicotinamide Adenine Dinucleotide Phosphate, Faʻaitiitiga Form Tetrasodium Salt)
Numera CAS: 2646-71-1
Fuafuaga mole: C21H26N7Na4O17P3
Molecular Weight: 833.35
Mama: ≥97% (HPLC)
Fa'aaliga: Pa'epa'e i le pa'epa'e pa'epa'e
Soluble: Solu fua ile vai (50 mg/mL)
Vaega Autu
- Mamalu Maualuga & Mausalī
- Synthetically maua mai ma ≥97% mama, faʻamautinoa le faʻatuatuaina faʻatinoga i suʻesuʻega maaleale biochemical.
- Faʻamau i le -20°C pe a teuina mago ma puipuia mai le malamalama; O vaifofo ua uma ona saunia e mafai ona fa'avasega ma teu i le -20°C mo le 1-2 masina.
- Talosaga Lautele
- Electron Donor: E galue o se cofactor mo oxidoreductases, e aofia ai le nitric oxide synthase ma le thioredoxin reductase.
- Biosynthesis: Taua mo le lipid, cholesterol, ma le nucleotide synthesis e ala i tali faʻaitiitia.
- Antioxidant Defense: Puipuia sela mai ituaiga o okesene reactive (ROS) e ala i le faatumauina o le faaitiitia o le glutathione.
- Su'esu'ega Su'esu'e: Fa'aaogaina i su'ega enzymatic mo su'esu'ega fa'afoma'i ma le atina'eina o vaila'au.
- Meatotino Mata
- Ole maualuga ole absorption ole UV ile 260 nm (ε = 15.0 × 10³ L·mol⁻¹·cm⁻¹) ma le 340 nm (ε = 6.3 × 10³ L·mol⁻¹·cm⁻¹), lelei mo spectrophotometric quantification .
Teuina & Taulimaina
- Teuina:
- Pu'upu'u: 2–8°C i totonu o atigipusa malu-malamalama .
- Taimi umi: -20°C i tulaga mago; aloese mai ta'amilosaga fa'a'aisa .
- Sauniuniga:
- Toe fa'apipi'i i totonu ole pa'u alkaline (fa'ata'ita'iga, 10 mM NaOH) mo le mautu lelei; vaifofo acidic fa'aleaga vave le NADPH .
- Centrifuge lyophilized paʻu i le 2,000-10,000 × g aʻo leʻi faʻaaogaina e faʻamautinoa ai le tutusa.
Saogalemu & Tausi
- Fa'amoemoega Fa'aoga: Mo na'o fa'amoemoega su'esu'e. E le mo suʻesuʻega, togafitiga, poʻo le taumafaina e tagata.
- Puipuiga o le Saogalemu:
- Fai ofutino falesuesue, totigilima, ma le puipuiga o mata i le taimi o le tagofiaina .
- E le lamatia i lalo o tulafono faatonutonu masani o felauaiga (UN NONH fa'avasegaina) .
Aisea e Filifilia ai a matou β-NADPH?
- Tulaga Fa'alelalolagi: Fa'atino i lalo ole FSSC22000 ma fa'atulafonoina fa'atonutonu lelei a le FDA.
- Lagolago Fa'atekinisi: Lagolagoina e le galulue fa'atasi ma fa'alapotopotoga ta'uta'ua (fa'ata'ita'iga, Iunivesite o Harvard, CAS) mo talosaga fa'apitoa .
- Fa'apipi'i Fa'apitoa: Maua i le 10 mg i le 1 g aofa'i e fetaui ma mana'oga fa'ata'ita'i eseese.
upu autu: β-NADPH, Coenzyme II faʻaititia,CAS 2646-71-1, foa'i eletise, oxidoreductase cofactor, NADPH tetrasodium masima
