Igama lemveliso:β-NADPH
Elinye igama:β-NADPH|beta-Nicotinamide adenine dinucleotide 2′-phosphate yehlisiwe tetrasodium ityuwa hydrate
Isithethantonye:beta-NADPH; I-2'-NADPH i-hydrate; I-Coenzyme II yanciphisa ityuwa ye-tetrasodium; Dihydronicotinamide adenine dinucleotide phosphate tetrasodium ityuwa; NADPH Na4; TPNH2 Na4; I-Triphosphopyridine nucleotide yanciphisa ityuwa ye-tetrasodium
Inombolo yeCAS: 2646-71-1
Inombolo ye-EINECS:220-163-3
Ubunyulu:≥98%
Ubushushu bokuGcina: -20°C
Imbonakalo: Umgubo omhlophe ukuya kumthubi
Inkcazo yeMveliso: β-NADPH (β-Nicotinamide Adenine Dinucleotide Phosphate, iFomu eNcitshisiweyo yeTetrasodium yeTyuwa)
Inombolo yeCAS: 2646-71-1
Ifomula yeemolekyuli: C21H26N7Na4O17P3
Ubunzima beMolekyuli: 833.35
Ubunyulu: ≥97% (HPLC)
Imbonakalo: Imhlophe ukuya kumgubo omhlophe
Ukunyibilika: Inyibilika ngokukhululekileyo emanzini (50 mg/mL)
Ezona mpawu
- Ukucoceka okuphezulu kunye nokuzinza
- Ithatyathwe ngokwenziwa ngo-≥97% ubunyulu, iqinisekisa ukusebenza okuthembekileyo kwiimvavanyo ezibuthathaka ze-biochemical.
- Izinzile kwi -20 ° C xa igcinwe yomile kwaye ikhuselwe ekukhanyeni; Izisombululo ezilungiselelwe kwangaphambili zinokukhutshwa kwaye zigcinwe kwi -20 ° C kwiinyanga ezi-1-2.
- Izicelo eziBanzi
- I-Electron Donor: Isebenza njenge-cofactor ye-oxidoreductases, kuquka i-nitric oxide synthase kunye ne-thioredoxin reductase.
- I-Biosynthesis: Ibaluleke kakhulu kwi-lipid, i-cholesterol, kunye ne-nucleotide synthesis ngokusabela kokunciphisa.
- Ukhuselo lwe-Antioxidant: Ikhusela iiseli ezivela kwiintlobo ze-oksijini esebenzayo (ROS) ngokugcina amanqanaba ancitshisiweyo e-glutathione.
- I-Diagnostic Reagents: Isetyenziswe kwiimvavanyo ze-enzymatic kuphando lweklinikhi kunye nophuhliso lwamayeza.
- IiPropathi zokubona
- Incochoyi yokufunxa kwe-UV kwi-260 nm (ε = 15.0 × 10³ L·mol⁻¹·cm⁻¹) kunye ne-340 nm (ε = 6.3 × 10³ L·mol⁻¹·cm⁻¹), ilungele i-spectrophotometric quantification
Ugcino & Nokuphatha
- Ugcino:
- Ixesha elifutshane: 2-8 ° C kwizikhongozeli ezingenamoya, ezikhuselwe ukukhanya.
- Ixesha elide: -20 ° C kwiimeko ze-desiccated; ziphephe imijikelo yokunyibilika komkhenkce .
- Ukulungiselela:
- Ukubuyisela kwakhona kwizithinteli ze-alkaline (umzekelo, i-10 mM NaOH) ukwenzela uzinzo oluphezulu; izisombululo ezineasidi zithomalalisa i-NADPH ngokukhawuleza.
- I-Centrifuge lyophilized powder kwi-2,000-10,000×g ngaphambi kokusetyenziswa ukuqinisekisa ukuba i-homogeneity.
Ukhuseleko kunye nokuThobela
- Usetyenziso olucetywayo: Ngeenjongo zophando kuphela. Ayenzelwa ukuxilonga, unyango, okanye ukusetyenziswa ngumntu .
- Ukhuseleko:
- Nxiba iidyasi zaselebhu, iiglavu, kunye nokukhusela amehlo ngexesha lokuphatha.
- Ayinabungozi phantsi kwemigaqo yezothutho esemgangathweni (uhlelo lwe-UN NONH) .
Kutheni ukhetha i-β-NADPH yethu?
- ImiGangatho yeHlabathi: Iveliswe phantsi kweFSSC22000 kunye ne-FDA-ithobela iinkqubo zokulawula umgangatho.
- INkxaso yobuGcisa: Ixhaswa yintsebenziswano namaziko aphambili (umzekelo, iYunivesithi yaseHarvard, iCAS) ukulungiselela izicelo eziphuma phambili.
- Ukupakishwa ngokweSiko: Ifumaneka kwi-10 mg ukuya kwi-1 g yobuninzi ukuze ihambelane neemfuno zovavanyo ezahlukeneyo.
Amagama angundoqo: β-NADPH, iCoenzyme II iyancipha,I-CAS 2646-71-1, i-electron donor, i-oxidoreductase cofactor, i-NADPH tetrasodium ityuwa
