Aha ngwaahịa: Tranexamic Acid 98% nke HPLC
Nọmba CAS:1197-18-8
Ụdị mkpụrụ ndụ: C₈H₁₅NO₂
Ibu ibu: 157.21 g/mol
Dị ọcha: ≥98% (HPLC)
Ọdịdị: White crystalline ntụ ntụ
Nchekwa: +4°C (obere oge), -20°C (ogologo oge)
Ngwa: Pharmaceutical, ịchọ mma, Nchọpụta
1. Nchịkọta ngwaahịa
A na-eji Tranexamic Acid (TXA), analog lysine sịntetik, dị ka ihe antifibrinolytic iji belata ọbara ọgbụgba na ntọala ịwa ahụ na trauma. Emepụtara ngwaahịa a n'okpuru njikwa mma siri ike, na-ahụ na ịdị ọcha nke ≥98% dị ka chromatography Liquid Liquid (HPLC) kwadoro. Ọdịdị kemịkal ya (trans-4- (aminomethyl) cyclohexanecarboxylic acid) na nkwụsi ike dị elu na-eme ka ọ dabara maka ngwa dị iche iche, gụnyere:
- Iji ọgwụ eme ihe: njikwa ọbara ọgbụgba, ọgwụgwọ ụbụrụ traumatic (TBI).
- Ihe ịchọ mma: ude na-achacha akpụkpọ ahụ na-achọ hyperpigmentation.
- Nnyocha: usoro nyocha na mmepe nke ọgwụ ọgwụ.
2. Chemical na anụ ahụ
- Aha IUPAC: 4- (Aminomethyl) cyclohexane-1-carboxylic acid
- Ọchị ọchị: NC[C@H] 1CCC @HC(=O)O
- Igodo InChI: inChi=1S/C8H15NO2/c9-5-6-1-3-7(4-2-6)8(10)11/h6-7H,1-5,9H2,(H,10,11)/t6-,7
- Ebe mgbaze: 386°C (dc.)
- Solubility: Soluble na mmiri (1N HCl, pH-buffers kwadoro), methanol, na acetonitrile.
3. Nkwenye mma
3.1 HPLC nyocha
Usoro HPLC anyị na-eme ka ọnụọgụgụ na ịkọwapụta adịghị ọcha:
- Ogidi: XBridge C18 (4.6 mm × 250 mm, 5 μm) ma ọ bụ otu.
- Usoro mkpanaaka: methanol: ihe nchekwa acetate (20 mM, pH 4) (75:25 v/v).
- Ọnụego ntinye: 0.8-0.9 ml/min.
- Nchọpụta: UV na 220 nm ma ọ bụ 570 nm (mwepụta post-derivatization na 1% ninhydrin).
- Ndabara Sistemu
- Nkenke: ≤2% CV maka mpaghara kacha elu (nsụgharị 6).
- Iweghachite: 98-102% (80%, 100%, 120% spiked ọkwa).
3.2 Profaịlụ adịghị ọcha
- adịghị ọcha A: ≤0.1%.
- adịghị ọcha B: ≤0.2%.
- Mkpokọta adịghị ọcha: ≤0.2%.
- Halides (dị ka Cl⁻): ≤140 ppm.
3.3 Nkwụsi ike
- pH Stability: Dakọtara na buffers (pH 2-7.4) na ngwọta IV nkịtị (dịka, fructose, sodium chloride).
- Thermal Stability: Stable na 37°C maka awa 24 na matrices ndu.
4. Ngwa
4.1 Iji ọgwụ eme ihe
- Nlekọta trauma: Na-ebelata onwu na ndị ọrịa TBI site na 20% (nnwale CRASH-3).
- Ịwa ahụ: Na-ebelata mfu ọbara perioperative (ọkpụkpụ, ịwa ahụ obi).
4.2 Ihe ịchọ mma
- Mechanism: Na-egbochi melanogenesis nke plasmin kpatara site na igbochi saịtị njikọ lysine.
- Nhazi: 3% TXA ude maka melasma na hyperpigmentation.
- Nchekwa: Iji ihe eme ihe na-ezere ihe egwu dị n'usoro (dịka ọmụmaatụ, thrombosis).
4.3 Nchọpụta & Mmepe
- Ụzọ nyocha:Synthesis: Prodrug interconversion ọmụmụ n'okpuru ọnọdụ acidic.
- UPLC-MS/MS: Maka nyocha plasma (LOD: 0.1 ppm).
- Fluorimetry: Mwepụta ya na NDA/CN (mmeghachi omume nkeji 5).
5. Nkwakọ ngwaahịa & Nchekwa
- Nkwakọ ngwaahịa mbụ: Akpa aluminom mechiri emechi nwere ihe eji eme ihe.
- Ndụ nchekwa: ọnwa 24 na -20 Celsius.
- Mbupu: Ọnọdụ okpomọkụ (nke kwadoro maka awa 72).
6. Nchekwa na nnabata
- Ijikwa: Jiri PPE (gloves, goggles) iji zere iku ume/kọntaktị.
- Ọnọdụ nchịkwa: kwekọrọ na USP, EP, na JP pharmacopeias.
- Nsi: LD₅₀ (ọnụ, oke)>5,000 mg/kg; na-abụghị carcinogenic.
7. Ntụaka
- Nkwenye dabara na sistemụ maka HPLC.
- Usoro nhazi nhazi na ntọhapụ.
- UPLC-MS/MS ntụnyere usoro.
- Ọnụ ọnụ ahịa na nlekọta trauma.
- Nkwụsi ike nhazi ihe ịchọ mma.
Keywords: Tranexamic Acid 98% HPLC, Antifibrinolytic Agent, Whitening Skin, Trauma Care, UPLC-MS/MS, CRASH-3 Trial, Melasma Treatment
Nkọwa meta: Tranexamic acid dị elu (≥98% nke HPLC) maka iji ahụike, ịchọ mma na nyocha. Ụzọ HPLC kwadoro, nlekọta trauma dị ọnụ ahịa, yana usoro isiokwu dị nchebe. CAS 1197-18-8.
