Igama Lomkhiqizo:I-Wogonin Bulk Powder

Umthombo Wezitshalo:Scutellaria baikalensis

Inombolo ye-CAS:632-85-9

Elinye Igama:Vogoni, wagonin, Wogonin hydrate, Vogonin Norwogonin 8-methyl ether

Imininingwane:≥98% HPLC

Umbala: Impushana ephuzi enephunga nokunambitha

Isimo se-GMO:GMO Mahhala

Ukupakisha: ku-25kgs fiber drums

Isitoreji: Gcina isitsha singavulwa endaweni epholile, eyomile, gwema ukukhanya okuqinile

Impilo Yeshelufu: Izinyanga ezingama-24 kusukela ngosuku lokukhiqiza

I-Scutellaria baikalensis iqukethe izakhi ezihlukahlukene zamakhemikhali, njenge-flavonoids ehlukahlukene, i-diterpenoids, i-polyphenols, ama-amino acid, amafutha ashisayo, i-sterol, i-benzoic acid, njalonjalo.Izimpande ezomile ziqukethe izinhlobo ezingaphezu kuka-110 zama-flavonoid njenge-baicalin, i-baicalein, i-wogonoside, ne-wogonin, okuyisithako esisebenzayo se-Scutellaria baikalensis.Ukukhishwa okujwayelekile okufana no-80% -90% HPLC Baicalin, 90% -98% HPLC Baicalein, 90% -95% HPLC Wogonoside, kanye 5% -98% HPLC Wogonin

I-In Vitro Activity:I-Wogonin ivimbela ukubonakaliswa kofuzo kwe-PMA-induced COX-2 ngokuvimbela isisho se-c-Jun kanye nokwenza kusebenze i-AP-1 kumaseli e-A549[1].I-Wogonin iyi-inhibitor ye-cyclin-dependent kinase 9 (CDK9) kanye ne-block phosphorylation yesizinda se-carboxy-terminal ye-RNA polymerase II ku-Ser.Ngakho-ke, kunciphisa ukuhlanganiswa kwe-RNA kanye nokwehla ngokushesha kwe-anti-apoptotic protein yesikhashana i-myeloid cell leukemia 1 (Mcl-1) okuholela ekungenweni kwe-apoptosis kumaseli omdlavuza.I-Wogonin ibophezela ngokuqondile ku-CDK9, cishe ku-ATP-binding pocketa futhi ayivimbeli i-CDK2, i-CDK4 ne-CDK6 kumithamo evimbela umsebenzi we-CDK9.I-Wogonin ivimbela ngokukhethekile i-CDK9 ku-malignant uma kuqhathaniswa nama-lymphocyte avamile.I-Wogonin futhi iyisinqamuleli esinamandla esikwazi ukuxosha i-?O2?[2].I-Wogonin ivimbela ngokuphawulekayo ukudluliselwa kwe-NFATc1 isuka ku-cytoplasm iye kuyi-nucleus kanye nomsebenzi wayo wokwenza kusebenze okubhaliwe.Iphinde ivimbele ngokuphawulekayo ukuhlukaniswa kwe-osteoclast futhi yehlise ukulotshwa kwe-osteoclast?ehambisana ne-immunoglobulin?njengesamukeli, i-tartrate?resistant acid phosphatase kanye ne-calcitonin receptor[4].I-Wogonin Ivimbela Umsebenzi we-N-acetyltransferase

Kumsebenzi we-Vivo:I-Wogonin icindezela ukukhula komdlavuza womuntu we-xenografts ku-vivo.Ngemithamo ebulalayo kumaseli wesimila, i-wogonin ayibonisi ubuthi obuncane noma obuncane kumaseli avamile futhi yayingenabo ubuthi obusobala ezilwaneni[2].I-Wogonin ingenza i-apoptosis ku-murine sarcoma S180 ngaleyo ndlela ivimbele ukukhula kwesimila kokubili ku-vitro kanye ne-vivo[3].Umjovo we-intraperitoneal we-200 mg/kg we-Wogonin ungavimbela ngokuphelele i-leukemia namaseli e-CEM

Ukuhlolwa kweseli:

Amaseli e-A549 ayisiko epuletini lemithombo engu-24 (amaseli angu-1.2×105/umthombo) usuku olungu-1 ngaphambi kokwelashwa kwe-wogonin.I-DMSO noma i-wogonin yengezwa kumaseli angu-A549 ihora elingu-1 ngaphambi kokukhuthazwa kwe-PMA, futhi amaseli afukanyelwa amanye amahora angu-6.Amaseli aqoqwa ngokwelashwa kwe-trypsin futhi izinombolo zamaseli zibalwa kusetshenziswa i-hemocytometer kanye nendlela yokukhishwa kwe-trypan eluhlaza okwesibhakabhaka.