Igama lomkhiqizo: I-Baohuoside I powder 98%

Umthombo we-Botanic:Epimedium koreanum Nakai, Epimedium brevicornu Maxim

I-CASNo:113558-15-9

Elinye Igama:I-Icariside-II,I-Icariin-II

Imininingwane:≥98%

Umbala:Okuphuzi Okukhanyayoimpushana enephunga sici nokunambitha

I-GMOIsimo:GMO Mahhala

Ukupakisha: ku-25kgs fiber drums

Isitoreji: Gcina isitsha singavulwa endaweni epholile, eyomile, gwema ukukhanya okuqinile

Impilo Yeshelufu: Izinyanga ezingama-24 kusukela ngosuku lokukhiqiza

I-Baohuoside I iyinhlanganisela ye-flavonoid etholakala ku-Epimedium koreanum.Njenge-inhibitor ye-CXCR4, ingavimbela inkulumo ye-CXCR4, ifake i-apoptosis, futhi inomsebenzi wokulwa ne-tumor.

Izimpushana ze-Baohuoside zitholakala ku-Epimedium koreanum Nakai noma i-Epimedium brevicornu Maxim, isitshalo sekhambi esidabuka e-China, e-Asia.Inqubo yokukhiqiza i-Baohuoside iqala ngezinto ezingavuthiwe ezivela esitshalweni se-Epimedium zichotshozwa bese zikhishwa nge-ethanol.Uketshezi olukhishiwe luyahlungwa futhi lugxiliswe ngaphambi kokuhlanjululwa ngamanzi futhi lube ne-enzymatic hydrolysis.Ngemva kwalokho, i-substance iyagezwa futhi ihlukaniselwe ku-ethanol, kulandelwa ukugxila, ukukhishwa kwe-solvent, ukubuyiswa kwe-solvent, i-crystallization, i-suction filtration, nokomiswa ekugcineni kukhiqiza i-Baohuoside powder 98% kwifomu yayo yempushana yokugcina.Kufanele kuqashelwe ngokucophelela isinyathelo ngasinye ngesikhathi sokucutshungulwa kwe-Baohuoside njengoba umsebenzi wayo othile usiza ekudaleni umkhiqizo ongagcina izinzuzo zawo zezempilo ngempumelelo kukho konke ukuphila kwawo kweshalofu uma ugcinwe kahle.Ekugcineni ukukhiqizwa kwe-Baohuoside kuveza isengezo esibalulekile esinohlu lwemiphumela emihle empilweni yomuntu uma sisetshenziswa ngendlela efanele.

In VitroUmsebenzi:I-Baohuoside I iyi-inhibitor ye-CXCR4 futhi yehlisa inkulumo ye-CXCR4 ku-12-25μ M. Baohuoside I (0-25μ M) ivimbela i-NF -κ Ukusebenza kwe-B ngendlela encike kumthamo futhi kuvimbela ukuhlasela okwenziwe yi-CXCL12 kwamangqamuzana omdlavuza womlomo wesibeletho.I-Bohorside iphinde ivimbele ukuhlasela kwamangqamuzana omdlavuza webele [1].I-Baohuoside I ivimbele ukusebenza kweseli ye-A549, ngamavelu e-IC50 angu-25.1μ M ngamahora angama-24, i-11.5μ M, futhi 9.6μ M ngamahora angama-48 namahora angama-72, ngokulandelana.I-Bohorside I (25μ M) ivimbela i-caspase cascade kumaseli e-A549, ikhuphule amazinga e-ROS, futhi yenze kusebenze ama-cascade okusayina i-JNK ne-p38MAPK [2].I-Boforseid I (3.125, 6.25, 12.5, 25.0, kanye no-50.0μ g/mL) kakhulu futhi kuncike kumthamo kuvimbele ukukhula kwamaseli e-esophageal squamous cell carcinoma Eca109, nge-IC50 ye-4.8μ g/mL amahora angu-48 [3].

Kumsebenzi we-Vivo:I-Baohuoside I (25 mg/kg) inganciphisa amazinga weβ - amaprotheni e-catenin kumagundane anqunu, Ukubonakaliswa kwe-cyclin D1 kanye ne-survivin

Ukuhlolwa kweseli:

Umthelela we-cytotoxic we-Baohuoside I kumaseli e-A549 unqunywe uhlolo lwe-MTT.Faka amaseli (1 × 10 4 cells/well) epuleti lomthombo elingu-96 bese uphatha nge-Baohua glycoside I (6.25, 12.5, kanye no-25 μ M) noma i-1mM NAC amahora angu-24, 48, noma angu-72.Ngemva kokukhipha isiko eliqukethe i-MTT, ncibilikisa amakristalu akhiwe ngokungeza i-DMSO emthonjeni ngamunye.Ngemva kokuxuba, linganisa ukumuncwa kwamaseli ku-540 nm usebenzisa i-Multiskan Spectrum Microplate Reader [2].

Ukuhlolwa kwezilwane:

Amagundane e-Female Balb/c (amaviki angu-4-6 ubudala) asetshenziselwa ukulinganisa.Vuna amaseli e-Eca109 Luc kusukela ku-sub confluence futhi uwamise kabusha ku-PBS kuze kube ukuminyana kokugcina kungu-2 × 107 amaseli/mL.Ngaphambi komjovo, misa kabusha amaseli ku-PBS futhi uwahlaziye usebenzisa i-0.4% ye-trypan eluhlaza okwesibhakabhaka yokukhishwa assay (amaseli abukhoma>90%).Ngomjovo ongaphansi kwesikhumba, amaseli e-1 × 107 Eca109 Luc asuka ku-200 μ LPBS ajovwe esiswini esingakwesokunxele segundane ngalinye kusetshenziswa inaliti engu-27G.Ngemva kwesonto elilodwa lomjovo weseli yesimila, i-Boforside I (25mg/kg igundane ngalinye) yajova esilonda kanye ngosuku, kuyilapho amagundane ayi-10 asetshenziselwa ukwelashwa kwe-vector anikezwa umthamo olinganayo we-PBS [3].