Khoom npe: Baohuoside I hmoov 98%

Botanic Source: Epimedium koreanum Nakai, Epimedium brevicornu Maxim

CASNo:113558-15-9

Lwm Lub Npe:Icariside- II,Icariin- II

Specifications: ≥98%

Xim:Lub teeb dajhmoov nrog cov yam ntxwv tsw thiab saj

GMOStatus: GMO Dawb

Ntim: hauv 25kgs fiber ntau nruas

Cia: Khaws lub thawv tsis qhib hauv qhov chaw txias, qhuav, Khaws kom deb ntawm lub teeb muaj zog

Lub neej txee: 24 lub hlis txij li hnub tsim khoom

Baohuoside I yog ib qho flavonoid compound tau los ntawm Epimedium koreanum.Raws li tus inhibitor ntawm CXCR4, nws muaj peev xwm inhibit qhov kev qhia ntawm CXCR4, induce apoptosis, thiab muaj kev tiv thaiv qog.

Baohuoside hmoov yog muab los ntawm Epimedium koreanum Nakai los yog Epimedium brevicornu Maxim, tshuaj ntsuab nroj tsuag nyob rau Suav teb, Asia.Cov txheej txheem tsim Baohuoside pib nrog cov khoom siv raw los ntawm Epimedium cog thiab tom qab ntawd muab rho tawm nrog ethanol.Cov kua muab rho tawm yog lim thiab concentrated ua ntej diluting nrog dej thiab nkag mus rau enzymatic hydrolysis.Tom qab ntawd, cov khoom yog ntxuav thiab parsed rau hauv ethanol, ua raws li cov concentration, hnyav extraction, hnyav rov qab, crystallization, nqus pom, thiab ziab uas thaum kawg ua rau Baohuoside hmoov 98% nyob rau hauv nws daim ntawv kawg hmoov.Ua tib zoo saib xyuas yuav tsum tau them rau txhua kauj ruam thaum ua haujlwm Baohuoside vim tias lawv cov haujlwm tshwj xeeb pab tsim cov khoom lag luam uas tuaj yeem khaws nws cov txiaj ntsig kev noj qab haus huv thoob plaws hauv nws lub txee lub neej thaum khaws cia kom zoo.Thaum kawg Baohuoside manufacturing yields ib qho tseem ceeb ntxiv nrog rau ntau yam ntawm cov txiaj ntsig zoo rau tus neeg noj qab haus huv thaum siv kom raug.

In VnwsKev ua si: Baohuoside I yog ib qho inhibitor ntawm CXCR4 thiab downregulates CXCR4 qhia ntawm 12-25μ M. Baohuoside I (0-25μ M) inhibit NF -κ B activation nyob rau hauv ib koob tshuaj-nyob ntawm tus yam ntxwv thiab inhibits CXCL12 induced invasion ntawm lub ncauj tsev menyuam hlwb.Bohorside kuv kuj inhibit qhov ntxeem tau ntawm cov qog nqaij hlav cancer mis [1].Baohuoside I inhibited A549 cell viability, nrog IC50 qhov tseem ceeb ntawm 25.1μ M 24 teev, 11.5μ m 9,6 uaμ M ntawm 48 teev thiab 72 teev, feem.Bohorside I (25μ M) inhibits caspase cascade nyob rau hauv A549 hlwb, nce ROS theem, thiab activates JNK thiab p38MAPK signaling cascades [2].Boforseid I (3.125, 6.25, 12.5, 25.0, thiab 50.0)μ g / mL) ntau thiab koob tshuaj tiv thaiv kev loj hlob ntawm esophageal squamous cell carcinoma Eca109 hlwb, nrog IC50 ntawm 4.8μ g / ml ntawm 48 teev [3].

Hauv Vivo Kev Ua Si: Baohuoside I (25 mg / kg) tuaj yeem txo cov qib ntawmβ - catenin protein nyob rau hauv cov nas liab qab, Kev qhia ntawm cyclin D1 thiab survivin

Kev sim ntawm tes:

Cov nyhuv cytotoxic ntawm Baohuoside I ntawm A549 hlwb tau txiav txim los ntawm MTT kev soj ntsuam.Inoculate cells (1 × 10 4 hlwb / zoo) rau hauv 96 lub phaj zoo thiab kho nrog Baohua glycoside I (6.25, 12.5, thiab 25 μ M) lossis 1mM NAC rau 24, 48, lossis 72 teev.Tom qab tshem tawm cov kab lis kev cai nruab nrab uas muaj MTT, yaj cov pob zeb tsim los ntawm kev ntxiv DMSO rau txhua qhov dej.Tom qab sib tov, ntsuas qhov nqus ntawm cov hlwb ntawm 540 nm siv Multiskan Spectrum Microplate Reader [2].

Tsiaj kev sim:

Poj niam Balb/c liab qab nas (4-6 lub lis piam) tau siv rau kev ntsuas.Sau Eca109 Luc hlwb los ntawm sub confluence thiab resuspend lawv nyob rau hauv PBS mus txog rau thaum kawg ceev yog 2 × 107 hlwb / mL.Ua ntej txhaj tshuaj, rov tso cov hlwb hauv PBS thiab txheeb xyuas lawv siv 0.4% trypan xiav cais tawm (cov hlwb nyob> 90%).Rau kev txhaj tshuaj subcutaneous, 1 × 107 Eca109 Luc hlwb los ntawm 200 μ LPBS tau txhaj rau hauv lub plab sab laug ntawm txhua tus nas uas siv 27G koob.Tom qab ib lub lim tiam ntawm cov qog cell txhaj, Boforside I (25mg / kg ib nas) tau txhaj rau hauv qhov mob ib hnub ib zaug, thaum 10 nas siv rau kev kho vector tau muab qhov sib npaug ntawm PBS [3].