Product Name: Baohuoside I poda 98%
Botanic Source:Epimedium koreanum Nakai, Epimedium brevicornu Maxim
CASNo:113558-15-9
Rimwe Zita:Icariside-II,Icariin-II
Zvinodiwa:≥98%
Color:Chiedza Yellowhupfu nehunhu hwema uye kuravira
GMOMamiriro:GMO Yemahara
Kurongedza: mune 25kgs fiber zvigubhu
Kuchengeta: Chengetedza mudziyo usina kuvhurwa munzvimbo inotonhorera, yakaoma, Chengetedza kure nechiedza chakasimba
Sherufu Hupenyu: Mwedzi makumi maviri nemana kubva pazuva rekugadzirwa
Baohuoside I musanganiswa weflavonoid unowanikwa kubva kuEpimedium koreanum.Se inhibitor yeCXCR4, inogona kuvharidzira kutaura kweCXCR4, kuunza apoptosis, uye ine anti-tumor basa.
Baohuoside powders anobva kuEpimedium koreanum Nakai kana Epimedium brevicornu Maxim, chirimwa chemishonga chinowanikwa kuChina, Asia.Iyo Baohuoside yekugadzira maitiro inotanga neiyo mbishi kubva kuEpimedium chirimwa ichipwanyika uye yozoburitswa ne ethanol.Mvura yakabviswa inosefa uye yakanyudzwa isati yanyungudutswa nemvura uye nekuita enzymatic hydrolysis.Mushure mezvo, chinhu chacho chinoshambidzwa uye chinoparidzirwa mu ethanol, inoteverwa nekugadzirisa, kunyungudutsa, kugadzirisa kugadzirisa, crystallization, suction filtration, uye kuomeswa izvo zvinozobudisa Baohuoside powder 98% muhupfu hwekupedzisira.Kuchenjerera kunofanirwa kupihwa kune yega yega nhanho panguva yeBaohuoside kugadzirisa sezvo basa ravo chairo rinobatsira kugadzira chigadzirwa chinogona kuchengetedza hutano hwacho musherufu muhupenyu hwayo hwose kana chachengetwa nemazvo.Pakupedzisira kugadzirwa kweBaohuoside kunopa imwe yakakosha yekuwedzera ine huwandu hwemhedzisiro yakanaka pahutano hwemunhu kana ikashandiswa nemazvo.
In VitroChiitiko:Baohuoside Ini inhibitor yeCXCR4 uye inoderedza kutaura kweCXCR4 pa12-25μ M. Baohuoside I (0-25μ M) inhibits NF -κ B kushandiswa mune imwe nzira-inotenderera uye inhibits CXCL12 inokonzerwa kupinda kwemasero emukenza wekervical.Bohorside ini ndinodzivisawo kupinda kwemasero ekenza yemazamu [1].Baohuoside I inhibited A549 cell viability, ine IC50 values dze25.1μ M pamaawa makumi maviri nemana, 11.5μ M uye 9.6μ M pa48 maawa uye 72 maawa, zvichiteerana.Bohorside I (25μ M) inhibits cascade cascade muA549 masero, inowedzera ROS mazinga, uye inomutsa JNK uye p38MAPK inosaina macascades [2].Boforseid I (3.125, 6.25, 12.5, 25.0, uye 50.0μ g/mL) zvakanyanya uye nedosi-yakavharira kukura kwesophageal squamous cell carcinoma Eca109 masero, ane IC50 ye4.8μ g/mL pamaawa 48 [3].
MuVivo Chiitiko:Baohuoside I (25 mg / kg) inogona kuderedza mazinga eβ - catenin protein mu nude mice, Kutaura kwe cyclin D1 uye kupona
Miedzo yemasero:
Iyo cytotoxic maitiro eBaohuoside I pamasero eA549 akatemerwa neMTT assay.Inoculate masero (1 × 10 4 masero / tsime) kupinda 96 tsime ndiro uye kubata Baohua glycoside I (6.25, 12.5, uye 25 μ M) kana 1mM NAC kwe24, 48, kana 72 maawa.Mushure mekubvisa tsika yepakati ine MTT, nyungudutsa makristasi akaumbwa nekuwedzera DMSO kune rimwe nerimwe tsime.Mushure mekusanganisa, pima kunyura kwemasero pa540 nm uchishandisa Multiskan Spectrum Microplate Reader [2].
Zviyedzo zvemhuka:
Mukadzi Balb / c nude mice (4-6 mavhiki ekuberekwa) akashandiswa kuyera.Kohwa Eca109 Luc masero kubva kusub confluence uye woamisa zvakare muPBS kusvika kuzara kwekupedzisira kwaita 2 × 107 maseru/mL.Usati wabaya jekiseni, misazve maseru muPBS woaongorora uchishandisa 0.4% trypan yeblue exclusion assay (live cells>90%).Nejekiseni rezasi, 1 × 107 Eca109 Luc masero kubva ku200 μ LPBS akabayiwa mudumbu rekuruboshwe rembeva yega yega achishandisa tsono ye27G.Mushure mevhiki imwe yebundu cell jekiseni, Boforside I (25mg/kg pagonzo) yakabayiwa muchironda kamwe chete pazuva, nepo 10 mbeva dzaishandiswa kurapa vector dzakapihwa huwandu hwakaenzana hwePBS [3].
