Aha ngwaahịa:Wogonin nnukwu ntụ ntụ

Isi mmalite nke ihe ọkụkụ: Scutellaria baicalensis

CAS Mba:632-85-9

Aha ọzọ:Vogoni, wagonin, Wogonin hydrate, Vogonin Norwogonin 8-methyl ether

Nkọwapụta:≥98% HPLC

Agba: Yellow ntụ ntụ nwere àgwà isi na uto

Ọnọdụ GMO: GMO efu

Mbukota: na 25kgs fiber drum

Nchekwa: Debe akpa na-emegheghị n'ebe dị jụụ, ebe kpọrọ nkụ, zere ọkụ siri ike

Ndụ shelf: ọnwa 24 site na ụbọchị mmepụta

Scutellaria baicalensis nwere ụdị kemịkal dị iche iche, dị ka flavonoids dị iche iche, diterpenoids, polyphenols, amino acids, mmanụ na-agbanwe agbanwe, sterol, benzoic acid, na ndị ọzọ.Mgbọrọgwụ nkụ nwere ihe karịrị ụdị flavonoids 110 dị ka baicalin, baicalein, wogonoside, na wogonin, bụ isi ihe na-arụ ọrụ nke Scutellaria baicalensis.Mwepụta ahaziri dị ka 80% -90% HPLC Baicalin, 90% -98% HPLC Baicalein, 90% -95% HPLC Wogonoside, na 5% -98% HPLC Wogonin

Na Ọrụ Vitro: Wogonin na-egbochi PMA-induced COX-2 gene expression site na igbochi okwu c-Jun na AP-1 na-arụ ọrụ na sel A549[1].Wogonin bụ onye na-egbochi cyclin-based kinase 9 (CDK9) yana igbochi phosphorylation nke ngalaba carboxy-terminal nke RNA polymerase II na Ser.N'ihi ya, ọ na-ebelata njikọ RNA na emesịa na-agbada ngwa ngwa nke protin anti-apoptotic myeloid cell leukemia 1 (Mcl-1) nke na-ebute apoptosis n'ime mkpụrụ ndụ cancer.Wogonin na-ejikọta ya na CDK9 ozugbo, ikekwe na ATP-jide pocketa na anaghị egbochi CDK2, CDK4 na CDK6 na doses na-egbochi ọrụ CDK9.Wogonin na-egbochi CDK9 nke ọma n'ịdị njọ ma e jiri ya tụnyere lymphocytes nkịtị.Wogonin bụkwa ihe na-egbochi oxidant nwere ike ịbọ ọbọ ?O2?[2].Wogonin na-egbochi mkpagharị nke NFATc1 site na cytoplasm gaa na nucleus na ọrụ ntinye akwụkwọ ya.Ọ na-egbochi oke osteoclast iche ma na-ebelata ederede osteoclast?metụtara immunoglobulin?dị ka onye na-anabata ya, tartrate?na-eguzogide acid phosphatase na calcitonin receptor[4].Wogonin na-egbochi ọrụ N-acetyltransferase

Na Ọrụ Vivo: Wogonin na-egbochi uto nke ọrịa cancer mmadụ xenografts na vivo.Na doses na-egbu mkpụrụ ndụ etuto ahụ, wogonin na-egosi enweghị ma ọ bụ ntakịrị nsi maka sel nkịtị yana enweghị nsi doro anya na anụmanụ[2].Wogonin nwere ike ịkpalite apoptosis na murine sarcoma S180 si otú a na-egbochi uto tumor ma na vitro ma na vivo[3].Ngwunye intraperitoneal nke 200 mg / kg Wogonin nwere ike igbochi leukemia na sel CEM kpamkpam.

Nnwale cell:

Mkpụrụ ndụ A549 bụ omenala na efere 24-well (1.2 × 105 cell / well) 1 ụbọchị tupu ọgwụgwọ wogonin.A na-agbakwunye DMSO ma ọ bụ wogonin n'ime sel A549 1 h tupu mkpali PMA, a na-etinyekwa mkpụrụ ndụ maka awa 6 ọzọ.A na-anakọta mkpụrụ ndụ site na ọgwụgwọ trypsin, a na-agụkwa ọnụọgụ cell site na iji hemocytometer na usoro iwepụ blue trypan.