Aha ngwaahịa: Baohuoside I ntụ ntụ 98%
Isi mmalite nke ihe ọkụkụ:Epimedium koreanum Nakai, Epimedium brevicornu Maxim
CASNo:113558-15-9
Aha ọzọ:Icariside-II,Icariin-II
Nkọwapụta:≥98%
Agba:Ọcha odontụ ntụ na njirimara isi na uto
GMOỌnọdụ: GMO efu
Mbukota: na 25kgs fiber drum
Nchekwa: Debe akpa na-emegheghị n'ebe dị jụụ, ebe kpọrọ nkụ, zere ọkụ siri ike
Ndụ shelf: ọnwa 24 site na ụbọchị mmepụta
Baohuoside I bụ ngwakọta flavonoid enwetara site na Epimedium koreanum.Dị ka onye na-egbochi CXCR4, ọ nwere ike igbochi nkwupụta nke CXCR4, mee ka apoptosis pụta, ma nwee ọrụ mgbochi tumor.
A na-enweta ntụ ntụ Baohuoside site na Epimedium koreanum Nakai ma ọ bụ Epimedium brevicornu Maxim, ahịhịa ahịhịa sitere na China, Eshia.Usoro nrụpụta Baohuoside na-amalite site na akụrụngwa sitere na osisi Epimedium nke a na-egwepịa wee were ethanol wepụta ya.A na-enyocha mmiri mmiri a na-amịpụta ma tinye ya n'ọrụ tupu ya etinye ya na mmiri ma na-enweta enzymatic hydrolysis.Mgbe nke ahụ gasịrị, a na-asacha ihe ahụ ma tụba ya na ethanol, na-esote ntinye uche, mmịpụta ihe mgbaze, mgbake mgbaze, crystallization, suction filtration, na ihicha nke na-emecha mepụta Baohuoside ntụ ntụ 98% n'ụdị ntụ ntụ ikpeazụ.A ga-akwụrịrị nlebara anya nke ọma na nzọụkwụ ọ bụla n'oge nhazi Baohuoside n'ihi na ọrụ ha na-enyere aka ịmepụta ngwaahịa nke nwere ike ijigide uru ahụike ya nke ọma n'oge ndụ ya niile mgbe echekwara ya nke ọma.N'ikpeazụ Baohuoside n'ichepụta na-ewepụta ihe mgbakwunye dị mkpa nwere ọtụtụ mmetụta dị mma na ahụike mmadụ mgbe ejiri ya nke ọma.
In VitroIhe omume:Baohuoside I bụ onye na-egbochi CXCR4 ma na-agbada okwu CXCR4 na 12-25μ M. Baohuoside I (0-25μ M) na-egbochi NF -κ Nkwalite B n'ụzọ dabere na dose ma gbochie CXCL12 mbuso agha nke mkpụrụ ndụ kansa cervical.Bohorside m na-egbochikwa mwakpo nke mkpụrụ ndụ kansa ara [1].Baohuoside I gbochiri ike ndụ cell A549, yana ụkpụrụ IC50 nke 25.1μ M na awa 24, 11.5μ M, na 9.6μ M na awa 48 na awa 72, n'otu n'otu.Bohorside I (25μ M) na-egbochi caspase cascade na mkpụrụ ndụ A549, na-abawanye ọkwa ROS, ma mee ka JNK na p38MAPK na-egosi cascades [2].Boforseid I (3.125, 6.25, 12.5, 25.0, na 50.0)μ g/mL) nke ukwuu na dose-dabere na-egbochi uto nke mkpụrụ ndụ squamous cell carcinoma esophageal Eca109, yana IC50 nke 4.8.μ g/ml na awa 48 [3].
Na ọrụ Vivo:Baohuoside I (25 mg/kg) nwere ike ibelata ọkwa nkeβ - protein catenin na ụmụ oke gba ọtọ, Okwu nke cyclin D1 na survivin
Nnwale cell:
Mmetụta cytotoxic nke Baohuoside I na sel A549 bụ nke MTT nyocha kpebiri.Inoculate cell (1 × 10 4 cell / well) n'ime efere ọma 96 ma na-emeso Baohua glycoside I (6.25, 12.5, na 25 μ M) ma ọ bụ 1mM NAC maka awa 24, 48, ma ọ bụ 72.Mgbe ị wepụsịrị usoro omenala nwere MTT, gbazee kristal ndị a kpụrụ site na ịgbakwunye DMSO na olulu mmiri ọ bụla.Mgbe agwakọtachara, tụọ nnabata nke mkpụrụ ndụ na 540 nm site na iji Multiskan Spectrum Microplate Reader [2].
Nnwale anụmanụ:
A na-eji ụmụ oke nwoke ịgba ọtọ Balb/c (izu 4-6) mee nha.Owuwe ihe ubi Eca109 Luc mkpụrụ ndụ site na sub confluence ma kwughachi ha na PBS ruo mgbe njupụta ikpeazụ bụ 2 × 107 sel/mL.Tupu ịgba ọgwụ, maliteghachi mkpụrụ ndụ ndị dị na PBS wee nyochaa ha site na iji 0.4% trypan blue exclusion assay (sel ndụ>90%).Maka ntụtụ subcutaneous, 1 × 107 Eca109 Luc cell sitere na 200 μ LPBS ka a na-agbanye n'ime afọ aka ekpe nke òké ọ bụla site na iji agịga 27G.Mgbe otu izu nke ịgba ọgwụ mgbochi ọrịa tumor cell, Boforside I (25mg/kg kwa òké) ka agbaba n'ime ọnya ahụ otu ugboro n'ụbọchị, ebe e nyere ụmụ oke iri eji agwọ ọrịa vector nha nha nke PBS [3].
