Dzina lazogulitsa:Wogonin Bulk Powder
Gwero la Botanic: Scutellaria baikalensis
Nambala ya CAS:632-85-9
Dzina Lina:Vogoni, wagonin, Wogonin hydrate, Vogonin Norwogonin 8-methyl ether
Zofotokozera:≥98% HPLC
Utoto: Ufa wachikasu wokhala ndi fungo komanso kukoma kwake
Mkhalidwe wa GMO: GMO Yaulere
Kulongedza: mu 25kgs fiber ng'oma
Kusungirako: Sungani chidebe chosatsegulidwa pamalo ozizira, owuma, Khalani kutali ndi kuwala kwamphamvu
Shelf Life: Miyezi 24 kuyambira tsiku lopangidwa
Scutellaria baikalensis lili zosiyanasiyana zigawo zikuluzikulu mankhwala, monga flavonoids zosiyanasiyana, diterpenoids, polyphenols, amino zidulo, kosakhazikika mafuta, sterol, asidi benzoic, ndi zina zotero.Mizu yowuma imakhala ndi mitundu yopitilira 110 ya flavonoids monga baicalin, baicalein, wogonoside, ndi wogonin, zomwe ndizomwe zimagwira ntchito pa Scutellaria baikalensis.Zolemba zokhazikika monga 80% -90% HPLC Baicalin, 90% -98% HPLC Baicalein, 90% -95% HPLC Wogonoside, ndi 5% -98% HPLC Wogonin
Mu Vitro Activity: Wogonin imaletsa PMA-induced COX-2 gene expression poletsa c-Jun expression ndi AP-1 activation mu A549 cell[1].Wogonin ndi inhibitor ya cyclin-dependent kinase 9 (CDK9) ndi block phosphorylation ya carboxy-terminal domain ya RNA polymerase II ku Ser.Chifukwa chake, imachepetsa kaphatikizidwe ka RNA ndipo kenako kutsika mwachangu kwa kanthawi kochepa ka anti-apoptotic protein myeloid cell leukemia 1 (Mcl-1) zomwe zimapangitsa kuti apoptosis alowe m'maselo a khansa.Wogonin imamangiriza mwachindunji ku CDK9, mwina ku pocketa yomangirira ATP ndipo sikuletsa CDK2, CDK4 ndi CDK6 pamiyeso yomwe imaletsa ntchito ya CDK9.Wogonin makamaka amalepheretsa CDK9 mu zoyipa poyerekeza ndi ma lymphocyte wamba.Wogonin ndi anti-oxidant yamphamvu yomwe imatha kuwononga ?O2?[2].Wogonin imalepheretsa kwambiri kusuntha kwa NFATc1 kuchokera ku cytoplasm kupita ku nucleus ndi ntchito yake yotsegula.Zimalepheretsanso kusiyanitsa kwa osteoclast komanso kumachepetsa kulembedwa kwa osteoclast?associated immunoglobulin?monga receptor, tartrate?resistant acid phosphatase ndi calcitonin receptor[4].Wogonin Imalepheretsa Ntchito ya N-acetyltransferase
Mu Vivo Activity: Wogonin imachepetsa kukula kwa khansa ya anthu xenografts mu vivo.Pamiyeso yakupha ku maselo otupa, wogonin samawonetsa poizoni kapena pang'ono kawopsedwe wa maselo abwinobwino komanso analibe poizoni wodziwikiratu pa nyama[2].Wogonin angapangitse apoptosis mu murine sarcoma S180 potero amalepheretsa kukula kwa chotupa mu vitro komanso mu vivo[3].Jekeseni wa intraperitoneal wa 200 mg/kg Wogonin atha kuletsa kwathunthu leukemia ndi ma cell a CEM.
Kuyesera kwa ma cell:
Maselo a A549 ndi chikhalidwe mu mbale ya 24-chitsime (1.2 × 105 maselo / bwino) 1 tsiku pamaso pa chithandizo cha wogonin.DMSO kapena wogonin imawonjezedwa m'maselo a A549 1 h musanayambe kukondoweza kwa PMA, ndipo maselo amapangidwanso kwa 6 h.Maselo amasonkhanitsidwa ndi chithandizo cha trypsin ndipo manambala a cell amawerengedwa pogwiritsa ntchito hemocytometer ndi trypan blue exclusion njira.
