Dzina lazogulitsa: Baohuoside I ufa 98%

Gwero la Botanic:Epimedium koreanum Nakai, Epimedium brevicornu Maxim

CASNo:113558-15-9

Dzina Lina:Icariside-II,Icariin-II

Zofotokozera:≥98%

Mtundu:Yellow Yowalaufa ndi khalidwe fungo ndi kukoma

Mtengo wa GMOMkhalidwe: GMO Yaulere

Kulongedza: mu 25kgs fiber ng'oma

Kusungirako: Sungani chidebe chosatsegulidwa pamalo ozizira, owuma, Khalani kutali ndi kuwala kwamphamvu

Shelf Life: Miyezi 24 kuyambira tsiku lopangidwa

Baohuoside I ndi gulu la flavonoid lomwe limapezeka ku Epimedium koreanum.Monga inhibitor ya CXCR4, imatha kuletsa kufotokoza kwa CXCR4, kuyambitsa apoptosis, ndipo imakhala ndi anti-chotupa.

Mafuta a Baohuoside amachokera ku Epimedium koreanum Nakai kapena Epimedium brevicornu Maxim, chomera chazitsamba chomwe chimachokera ku China, Asia.Njira yopangira Baohuoside imayamba ndi zopangira kuchokera ku chomera cha Epimedium ndikuphwanyidwa ndikuchotsedwa ndi ethanol.The yotengedwa madzi amasefedwa ndi anaikira pamaso diluting ndi madzi ndi akukumana enzymatic hydrolysis.Pambuyo pake, chinthucho chimatsukidwa ndikuyikidwa mu ethanol, ndikutsatiridwa ndi ndende, zosungunulira m'zigawo, zosungunulira zosungunulira, crystallization, suction filtration, ndi kuyanika zomwe pamapeto pake zimapanga ufa wa Baohuoside 98% mu mawonekedwe ake omaliza a ufa.Chisamaliro chiyenera kuperekedwa pa sitepe iliyonse panthawi yokonza Baohuoside chifukwa ntchito yake imathandizira kupanga mankhwala omwe amatha kusunga ubwino wake wathanzi nthawi yonse ya alumali pamene asungidwa bwino.Pamapeto pake kupanga Baohuoside kumabweretsa chowonjezera chofunikira chokhala ndi zotsatira zabwino paumoyo wa munthu zikagwiritsidwa ntchito moyenera.

In ViziZochita:Baohuoside I ndi inhibitor ya CXCR4 ndipo imatsitsa mawu a CXCR4 pa 12-25μ M. Baohuoside I (0-25μ M) amaletsa NF -κ B kuyambitsa m'njira yodalira mlingo ndikuletsa CXCL12 kuyambitsa kuukira kwa maselo a khansa ya khomo lachiberekero.Bohorside ndimaletsanso kuwukira kwa ma cell a khansa ya m'mawere [1].Baohuoside I inaletsa mphamvu ya A549 cell, ndi IC50 values ​​of 25.1μ M pa maola 24, 11.5μ M, ndi 9.6μ M pa maola 48 ndi maola 72, motero.Bohorside I (25μ M) imalepheretsa cascade m'maselo a A549, imawonjezera milingo ya ROS, ndikuyambitsa ma signature a JNK ndi p38MAPK [2].Boforseid I (3.125, 6.25, 12.5, 25.0, ndi 50.0)μ g/mL) kwambiri ndipo modalira mlingo anatsekereza kukula kwa esophageal squamous cell carcinoma Eca109 maselo, ndi IC50 wa 4.8μ g/mL pa maola 48 [3].

Mu Vivo Ntchito:Baohuoside I (25 mg/kg) akhoza kuchepetsa milingo yaβ - mapuloteni a catenin mu mbewa zamaliseche, Mafotokozedwe a cyclin D1 ndi survivin

Kuyesera kwa ma cell:

Mphamvu ya cytotoxic ya Baohuoside I pa maselo a A549 idatsimikiziridwa ndi kuyesa kwa MTT.Inoculate maselo (1 × 10 4 maselo / bwino) mu 96 bwino mbale ndi kuchitira ndi Baohua glycoside I (6.25, 12.5, ndi 25 μ M) kapena 1mM NAC kwa 24, 48, kapena 72 hours.Mukachotsa chikhalidwe chomwe chili ndi MTT, sungunulani makhiristo opangidwa powonjezera DMSO pachitsime chilichonse.Mukasakaniza, yesani kuyamwa kwa maselo pa 540 nm pogwiritsa ntchito Multiskan Spectrum Microplate Reader [2].

Zoyeserera zanyama:

Akazi a Balb / c mbewa zamaliseche (masabata 4-6) adagwiritsidwa ntchito poyeza.Kololani ma cell a Eca109 Luc kuchokera ku sub confluence ndikuwatsitsimutsanso mu PBS mpaka kachulukidwe komaliza ndi 2 × 107 maselo/mL.Musanapereke jakisoni, ikaninso ma cell mu PBS ndikuwasanthula pogwiritsa ntchito 0.4% trypan blue exclusion assay (ma cell amoyo> 90%).Kwa jekeseni wa subcutaneous, 1 × 107 Eca109 Luc maselo ochokera ku 200 μ LPBS anabayidwa pamimba yakumanzere kwa mbewa iliyonse pogwiritsa ntchito singano ya 27G.Pambuyo pa sabata imodzi ya jakisoni wa cell chotupa, Boforside I (25mg/kg pa mbewa) anabayidwa mu chotupa kamodzi patsiku, pomwe mbewa 10 zomwe zimagwiritsidwa ntchito pochiza vekitala zidapatsidwa kuchuluka kwa PBS [3].