Magaca alaabta:Baohuoside I budada 98%

Isha dhirta:Epimedium koreanum Nakai, Epimedium brevicornu Maxim

CASNo:113558-15-9

Magac kale:Icariside-II,Icariin-II

Tilmaamaha:≥98%

Midabka:Jaalle khafiif ahbudo leh ur sifo iyo dhadhan

GMOXaalada:GMO Bilaash

Xirmooyinka: 25kgs durbaannada fiber-ka

Kaydinta: Weelka ha furin meel qabow oo qalalan, ka fogow iftiinka xooggan

Life Life:24 bilood laga bilaabo taariikhda wax soo saarka

Baohuoside I waa iskudhis flavonoid ah oo laga helay Epimedium koreanum.Sida horjoogaha CXCR4, waxay joojin kartaa muujinta CXCR4, waxay dhalisaa apoptosis, waxayna leedahay firfircooni ka hortagga burooyinka.

Budada Baohuoside waxaa laga soo qaatay Epimedium koreanum Nakai ama Epimedium brevicornu Maxim, oo ah warshad dhireed u dhalatay Shiinaha, Aasiya.Habka wax soo saarka Baohuoside wuxuu ku bilaabmaa alaabta ceeriin ee warshadda Epimedium oo la burburiyo ka dibna lagu soo saaro ethanol.Dareeraha la soo saaray waa la sifeeyaa oo la ururiyaa ka hor inta aan lagu milmin biyo oo aan la marin hayroolaysis enzymatic ah.Ka dib, walaxda waa la dhaqay oo lagu jeexjeexay ethanol, oo ay ku xigto xoojinta, soo saarista dareeraha, soo kabashada dareeraha, crystallization, sifeynta nuugista, iyo qalajinta taas oo ugu dambeyntii soo saarta budada Baohuoside 98% qaabkeeda ugu dambeeya.Feejignaan taxaddar leh waa in la siiyaa tillaabo kasta inta lagu jiro farsamaynta Baohuoside maaddaama shaqadooda gaarka ahi ay caawiso abuurista badeecad si wax ku ool ah u haysan karta faa'iidooyinkeeda caafimaad inta lagu jiro cimrigeeda shelf marka si habboon loo kaydiyo.Ugu dambeyntii wax soo saarka Baohuoside waxay soo saartaa kaab muhiim ah oo leh saameyn togan oo kala duwan oo ku saabsan caafimaadka qofka marka si sax ah loo isticmaalo.

In VitroDhaqdhaqaaqa:Baohuoside I waa xakameynta CXCR4 waxayna hoos u dhigtaa muujinta CXCR4 ee 12-25μ M. Baohuoside I (0-25μ M) waxay joojisaa NF-κ Dhaqdhaqaaqa B ee habka qiyaasta-ku-tiirsanaanta waxayna ka hortagtaa duulaanka CXCL12 ee unugyada kansarka ilmo-galeenka.Bohorside I sidoo kale waxay joojisaa duulaanka unugyada kansarka naasaha [1].Baohuoside I wuxuu joojiyay jiritaanka unugyada A549, oo leh IC50 qiyamka 25.1μ M saacada 24, 11.5μ M, iyo 9.6μ M at 48 saacadood iyo 72 saacadood, siday u kala horreeyaan.Bohorside I (25μ M) waxay joojisaa qulqulka caspase ee unugyada A549, waxay kordhisaa heerarka ROS, waxayna kicisaa JNK iyo p38MAPK calaamadaha cascades [2].Boforseid I (3.125, 6.25, 12.5, 25.0, iyo 50.0)μ g/mL) si weyn oo ku tiirsan qiyaasta ayaa xannibay koritaanka kansarka unugyada hunguriga Eca109 unugyada Eca109, oo leh IC50 ee 4.8μ g/ml saacada 48 [3].

Waxqabadka Vivo:Baohuoside I (25 mg/kg) waxay yarayn kartaa heerarkaβ - borotiinka catenin ee jiirarka qaawan, muujinta cyclin D1 iyo survivin

Tijaabooyinka unugga:

Saamaynta cytotoxic ee Baohuoside I ee unugyada A549 waxaa go'aamiyay MTT assay.Ku tallaal unugyo (1 × 10 4 unug / ceel) geli saxan si fiican 96 oo ku dawee Baohua glycoside I (6.25, 12.5, iyo 25 μM) ama 1mM NAC 24, 48, ama 72 saacadood.Ka dib markaad ka saarto dhexdhexaadinta dhaqanka ee ay ku jirto MTT, milaan muraayadaha la sameeyay adigoo ku daraya DMSO ceel kasta.Isku qas ka dib, qiyaas nuugista unugyada 540 nm adoo isticmaalaya Multiskan Spectrum Microplate Reader [2].

Tijaabooyinka xayawaanka:

Dumar Balb/c jiirar qaawan (4-6 toddobaad jir) ayaa loo isticmaalay cabbirka.Harvest Eca109 Unugyada Luc ee ka soo baxa isku dhafka hoose oo dib ugu celi PBS ilaa cufnaanta u dambaysa ay tahay 2 × 107 unug/mL.Kahor duritaanka ka hor, dib u celi unugyada PBS oo falanqee adoo isticmaalaya 0.4% trypan blue assay (unugyada nool>90%).Cirbad hoosaadka, 1 × 107 Eca109 Luc unugyada 200 μ LPBS ayaa lagu duray caloosha bidix ee jiir kasta iyadoo la isticmaalayo irbad 27G ah.Hal usbuuc ka dib cirbadeynta unugyada burooyinka, Boforside I (25mg/kg halkii jiir) ayaa lagu duray nabarka hal mar maalintii, halka jiirarka 10 ee loo isticmaalo daaweynta vector la siiyay mugga siman ee PBS [3].