Lebitso la Sehlahisoa: Baohuoside I phofo 98%
Mohloli oa Botanic:Epimedium koreanum Nakai, Epimedium brevicornu Maxim
CASNo:113558-15-9
Lebitso le Leng:Icariside-II,Icariin-II
Litlhaloso:≥98%
Mmala:Bosehla bo Khanyangphofo e nang le monko le tatso e ikhethang
GMOBoemo: GMO Mahala
Ho paka: ka meqomo ea fiber ea 25kgs
Polokelo: Boloka setshelo se sa buloa sebakeng se phodileng, se omileng, Qoba hole le leseli le matla
Shelf Life: Likhoeli tse 24 ho tloha ka letsatsi la tlhahiso
Baohuoside I ke motsoako oa flavonoid o fumanoang ho Epimedium koreanum.Joalo ka inhibitor ea CXCR4, e ka thibela polelo ea CXCR4, ea etsa hore apoptosis, 'me e na le ts'ebetso e khahlanong le hlahala.
Li-powders tsa Baohuoside li tsoa ho Epimedium koreanum Nakai kapa Epimedium brevicornu Maxim, semela sa litlama se tsoaletsoeng Chaena, Asia.Ts'ebetso ea tlhahiso ea Baohuoside e qala ka thepa e tala e tsoang semela sa Epimedium e silakanngoa ebe e ntšoa ka ethanol.Mokelikeli o ntšitsoeng oa tlhotloa le ho kenngoa pele o hlapolloa ka metsi 'me o etsoa enzymatic hydrolysis.Ka mor'a moo, ntho ena e hlatsuoa ebe e aroloa ka ethanol, e lateloa ke ho tsepamisa mohopolo, ho ntšoa ha solvent, ho hlaphoheloa ha solvent, crystallization, suction filtration, le ho omisa hoo qetellong ho hlahisang phofo ea Baohuoside 98% ka mokhoa oa eona oa ho qetela oa phofo.Tlhokomelo e 'ngoe le e 'ngoe e tlameha ho lefshoa mohatong o mong le o mong nakong ea ts'ebetso ea Baohuoside kaha ts'ebetso ea bona e thusa ho theha sehlahisoa se ka bolokang melemo ea bophelo bo botle bophelong bohle ba sona ha se bolokiloe hantle.Qetellong tlhahiso ea Baohuoside e fana ka tlatsetso ea bohlokoa e nang le mefuta e mengata ea litlamorao tse ntle bophelong ba motho ha e sebelisoa ka nepo.
In VitroKetsahalo:Baohuoside I ke inhibitor ea CXCR4 mme e theola polelo ea CXCR4 ho 12-25μ M. Baohuoside I (0-25μ M) thibela NF -κ B ts'ebetsong ka mokhoa o itšetlehileng ka tekanyo ea tekanyo le ho thibela ho hlaseloa ha CXCL12 ea lisele tsa mofetše oa popelo.Bohorside Ke boetse ke thibela tlhaselo ea lisele tsa mofetše oa matsoele [1].Baohuoside I e thibetse A549 ho sebetsa ha sele, ka IC50 boleng ba 25.1μ M ka lihora tse 24, 11,5μ M, le 9.6μ M ka lihora tse 48 le lihora tse 72, ka ho latellana.Bohorside I (25μ M) e thibela "caspase cascade" liseleng tsa A549, e eketsa maemo a ROS, 'me e kenya tšebetsong li-cascade tsa JNK le p38MAPK [2].Boforseid I (3.125, 6.25, 12.5, 25.0, le 50.0μ g/mL) haholo le ho itšetleha ka lethal dose thibela kholo ea esophageal squamous cell carcinoma lisele tsa Eca109, tse nang le IC50 ea 4.8μ g/mL ka lihora tse 48 [3].
Ts'ebetsong ea Vivo:Baohuoside I (25 mg/kg) e ka fokotsa maemo aβ - protheine ea catenin ka litoeba tse hlobotseng, Polelo ea cyclin D1 le survivin
Liteko tsa lisele:
Phello ea cytotoxic ea Baohuoside I liseleng tsa A549 e ile ea khethoa ke tlhahlobo ea MTT.Enoculate lisele (1 × 10 4 lisele / seliba) ka 96 seliba poleiti 'me tšoara le Baohua glycoside I (6.25, 12.5, le 25 μ M) kapa 1mM NAC bakeng sa 24, 48, kapa 72 lihora tse.Ka mor'a ho tlosa setso se nang le MTT, qhala likristale tse entsoeng ka ho eketsa DMSO selibeng ka seng.Ka mor'a ho kopanya, lekanya ho monya ha lisele ho 540 nm ho sebelisa Multiskan Spectrum Microplate Reader [2].
Liteko tsa liphoofolo:
Litoeba tse tšehali tsa Balb / c (libeke tse 4-6) li ne li sebelisetsoa ho lekanya.Kotula lisele tsa Eca109 Luc ho tloha sub confluence le ho li emisa hape ho PBS ho fihlela tekano ea ho qetela e le 2 × 107 cell/mL.Pele o entoa, emisa lisele tsa PBS hape 'me u li hlahlobe u sebelisa tlhahlobo ea 0.4% ea trypan blue exclusion assay (lisele tse phelang> 90%).Bakeng sa ente ea subcutaneous, 1 × 107 Eca109 Luc lisele tse tsoang 200 μ LPBS li ile tsa kenngoa ka mpeng e letšehali ea mouse ka 'ngoe ho sebelisa nale ea 27G.Ka mor'a beke e le 'ngoe ea ente ea lisele tsa tumor, Boforside I (25mg / kg ka mouse) e ile ea kenngoa ka leqeba hang ka letsatsi, ha litoeba tse 10 tse sebelisetsoang phekolo ea vector li ile tsa fuoa palo e lekanang ea PBS [3].
