Lebitso la Sehlahisoa: Tranexamic Acid 98% ka HPLC
Nomoro ea CAS:1197-18-8
Foromo ea molek'hule: C₈H₁₅NO₂
Boima ba 'mele: 157.21 g / mol
Bohloeki: ≥98% (HPLC)
Ponahalo: Phofo e tšoeu ea kristale
Polokelo: +4°C (nako e khutšoanyane), -20°C (nako e telele)
Kopo: Pharmaceutical, Cosmetics, Research
1. Tlhaloso ea Sehlahisoa
Tranexamic Acid (TXA), e leng analoge ea maiketsetso ea lysine, e sebelisoa haholo e le moemeli oa antifibrinolytic ho fokotsa ho tsoa mali maemong a ho buuoa le ho sithabela maikutlo. Sehlahisoa sena se entsoe ka taolo e thata ea boleng, ho netefatsa bohloeki ba ≥98% joalo ka ha ho netefalitsoe ke High-Performance Liquid Chromatography (HPLC). Sebopeho sa eona sa lik'hemik'hale (trans-4-(aminomethyl) cyclohexanecarboxylic acid) le botsitso bo phahameng li etsa hore e tšoanelehe bakeng sa lits'ebetso tse fapaneng, ho kenyelletsa:
- Tšebeliso ea Bongaka: Ho laola mali, phekolo ea kotsi e sithabetsang ea boko (TBI).
- Litlolo: Litlolo tse soeufatsang letlalo tse shebileng hyperpigmentation.
- Patlisiso: nts'etsopele ea mekhoa ea tlhahlobo le lithuto tsa pharmacokinetic.
2. Thepa ea Lik'hemik'hale le 'Mele
- Lebitso la IUPAC: 4-(Aminomethyl) cyclohexane-1-carboxylic acid
- MOSOSO: NC[C@@H]1CCC@HC(=O)O
- InChI Key: InChI=1S/C8H15NO2/c9-5-6-1-3-7(4-2-6)8(10)11/h6-7H,1-5,9H2,(H,10,11)/t6-,7
- Sebaka se qhibilihang: 386°C (Dec.)
- Ho qhibiliha: Ho qhibiliha ka metsing (1N HCl, li-buffers tse fetotsoeng pH), methanol, le acetonitrile.
3. Tiisetso ea Boleng
3.1 Tlhahlobo ea HPLC
Mokhoa oa rona oa HPLC o netefatsa palo e nepahetseng ea lintlha le profiling ea litšila:
- Kholomo: XBridge C18 (4.6 mm × 250 mm, 5 μm) kapa e lekanang.
- Mokhahlelo oa Mobile: Methanol:acetate buffer (20 mM, pH 4) (75:25 v/v).
- Sekhahla sa Phallo: 0.8-0.9 mL / min.
- Ho lemoha: UV ho 220 nm kapa 570 nm (post-derivatization le 1% ninhydrin).
- Ho Hlokahala Sistimi:
- Ho nepahala: ≤2% CV bakeng sa sebaka se phahameng (6 replicates).
- Pholiso: 98-102% (80%, 100%, 120% maemo a spiked).
3.2 Boemo ba Tšilafalo
- Ho se hloeke A: ≤0.1%.
- Ho se hloeke B: ≤0.2%.
- Kakaretso ea Litšila: ≤0.2%.
- Halides (joalo ka Cl⁻): ≤140 ppm.
3.3 Botsitso
- pH botsitso: E lumellana le li-buffers (pH 2-7.4) le litharollo tse tloaelehileng tsa IV (mohlala, fructose, sodium chloride).
- Thermal Stability: E tsitsitseng ho 37 ° C bakeng sa lihora tse 24 ho matrices a likokoana-hloko.
4. Likopo
4.1 Tšebeliso ea Bongaka
- Tlhokomelo ea Matšoenyeho: E fokotsa ho shoa ho bakuli ba TBI ka 20% (teko ea CRASH-3).
- Opereishene: E fokotsa tahlehelo ea mali ka mor'a opereishene (opereishene ea masapo, ea pelo).
4.2 Litlolo
- Mechanism: E thibela melanogenesis e bakoang ke plasmin ka ho thibela libaka tse tlamang lysine.
- Mefuta: 3% ea litlolo tsa TXA bakeng sa melasma le hyperpigmentation.
- Tšireletseho: Tšebeliso ea lihlooho e qoba likotsi tsa tsamaiso (mohlala, thrombosis).
4.3 Lipatlisiso le Ntlafatso
- Mekhoa ea ho Hlahlobisisa:Synthesis: Lithuto tsa ho fetolela lithethefatsi tlas'a maemo a acidic.
- UPLC-MS/MS: Bakeng sa tlhahlobo ea plasma (LOD: 0.1 ppm).
- Fluorimetry: Derivatization le NDA/CN (karabelo ea metsotso e 5).
5. Packaging & Storage
- Packaging ea mantlha: Mekotla ea aluminium e tiisitsoeng e nang le desiccant.
- Shelf Life: Likhoeli tse 24 ho -20°C.
- Thomello: Thempereichara ea tikoloho (e netefalitsoe ka lihora tse 72).
6. Polokeho le Boikamahanyo
- Ho tšoara: Sebelisa PPE (liatlana, likhalase) ho qoba ho hema/ho kopana.
- Boemo ba Taolo: E lumellana le USP, EP, le JP pharmacopeias.
- Chefo: LD₅₀ (molomo, rat)>5,000 mg/kg; e seng carcinogenic.
7. Litšupiso
- Netefatso ea ho nepahala ha sistimi bakeng sa HPLC.
- Calibration curve le derivatization protocols.
- UPLC-MS/MS mokhoa papiso.
- Tšebeliso ea litšenyehelo tlhokomelong ea mahlomola.
- Ho tsitsa ha sebopeho sa litlolo.
Mantsoe a bohlokoa: Tranexamic Acid 98% HPLC, Antifibrinolytic Agent, Skin Whitening, Trauma Care, UPLC-MS/MS, CRASH-3 Trial, Melasma Treatment
Tlhaloso ea Meta: High-purity Tranexamic Acid (≥98% ka HPLC) bakeng sa tšebeliso ea bongaka, litlolo, le lipatlisiso. Mekhoa e netefalitsoeng ea HPLC, tlhokomelo ea maqeba a kotsi, le mekhoa e sireletsehileng ea lihlooho. CAS 1197-18-8.
