Igama leMveliso:Baohuoside I powder 98%

Umthombo weBhotani:Epimedium koreanum Nakai, Epimedium brevicornu Maxim

CASNo:113558-15-9

Elinye Igama:Icariside-II,Icariin-II

Iinkcukacha:≥98%

Umbala:UKhanya Mthubiumgubo onevumba elibonakalayo kunye nencasa

GMOIsimo:GMO Mahala

Ukupakisha: kwi-25kgs fiber drums

Ugcino: Gcina isikhongozeli singavulwanga kwindawo epholileyo neyomileyo, Zigcine kude nokukhanya okuqinileyo

Ubomi beShelf: iinyanga ezingama-24 ukusuka kumhla wokuvelisa

I-Baohuoside I yi-compound ye-flavonoid efunyenwe kwi-Epimedium koreanum.Njenge-inhibitor ye-CXCR4, inokuthintela ukubonakaliswa kwe-CXCR4, yenza i-apoptosis, kwaye inomsebenzi wokuchasana ne-tumor.

I-Baohuoside powders ithathwe kwi-Epimedium koreanum Nakai okanye i-Epimedium brevicornu Maxim, isityalo semifuno esiphuma e-China, e-Asia.Inkqubo yokuvelisa i-Baohuoside iqala ngezinto eziluhlaza ezivela kwisityalo se-Epimedium zityunyuzwa kwaye zikhutshwe nge-ethanol.Ulwelo olukhutshiweyo luyahluzwa kwaye lugxininiswe phambi kokuba luxutywe ngamanzi kwaye lwenziwe nge-enzymatic hydrolysis.Emva koko, i-substance ihlanjwe kwaye ihlanjululwe kwi-ethanol, ilandelwa kukugxininiswa, ukutsalwa kwe-solvent, ukubuyiswa kwe-solvent, i-crystallization, i-suction filtration, kunye nokomisa ekugqibeleni kuvelisa i-Baohuoside powder 98% kwifom yayo yokugqibela.Ingqwalasela elumkileyo kufuneka ihlawulwe kwinyathelo ngalinye ngexesha lokulungiswa kwe-Baohuoside njengoko umsebenzi wabo othile unceda ukudala imveliso enokugcina ngokufanelekileyo izibonelelo zayo zempilo ebomini bayo bonke xa igcinwe ngokufanelekileyo.Ekugqibeleni imveliso ye-Baohuoside ivelisa isongezelelo esibalulekileyo esinoluhlu lweziphumo ezilungileyo kwimpilo yomntu xa isetyenziswe ngokuchanekileyo.

In VitroUmsebenzi:I-Baohuoside ndiyi-inhibitor ye-CXCR4 kwaye ibeka phantsi i-CXCR4 intetho kwi-12-25μ M. Baohuoside I (0-25μ M) inqanda iNF -κ Ukusebenza kwe-B ngendlela exhomekeke kwi-dose kwaye inqanda ukuhlaselwa kwe-CXCL12 eyenziwa ngamaseli omhlaza womlomo wesibeleko.I-Bohorside ndiyanqanda uhlaselo lweeseli zomhlaza webele [1].I-Baohuoside I inqanda ukusebenza kweseli ye-A549, kunye nexabiso le-IC50 le-25.1μ M kwiiyure ezingama-24, i-11.5μ M, kunye no-9.6μ M kwiiyure ze-48 kunye neeyure ze-72, ngokulandelanayo.IBohorside I (25μ M) inqanda i-caspase cascade kwiiseli ze-A549, inyusa amanqanaba e-ROS, kwaye isebenze i-JNK kunye ne-p38MAPK yokubonisa i-cascade [2].I-Boforseid I (3.125, 6.25, 12.5, 25.0, kunye no-50.0μ g/mL) kakhulu kwaye umthamo-ngokuxhomekeka kuthintelwe ukukhula esophageal squamous cell carcinoma iiseli Eca109, kunye IC50 of 4.8μ ig/mL kwiiyure ezingama-48 [3].

KuMsebenzi weVivo:Baohuoside I (25 mg / kg) inokunciphisa amanqanabaβ - iprotheni ye-catenin kwiimpuku ezinqunu, Ukubonakaliswa kwe-cyclin D1 kunye ne-survivin

Imifuniselo yeeseli:

Umphumo we-cytotoxic we-Baohuoside I kwiiseli ze-A549 zagqitywa ngovavanyo lwe-MTT.Inoculate iiseli (1 × 10 4 iiseli / kakuhle) ibe 96 ipleyiti kakuhle kwaye uphathe kunye Baohua glycoside I (6.25, 12.5, kunye 25 μ M) okanye 1mM NAC for 24, 48, okanye 72 iiyure.Emva kokususa i-culture medium equkethe i-MTT, chitha iikristale ezenziweyo ngokungeza i-DMSO kwiqula ngalinye.Emva kokuxuba, linganisa ukuxutywa kweeseli kwi-540 nm usebenzisa i-Multiskan Spectrum Microplate Reader [2].

Imifuniselo yezilwanyana:

I-Female Balb / c iigundane ezinqunu (iiveki ezi-4-6 ubudala) zazisetyenziselwa ukulinganisa.Ukuvuna iiseli ze-Eca109 ze-Luc ukusuka kwi-sub confluence kwaye uzimise kwakhona kwi-PBS de uxinano lokugqibela yi-2 × 107 iiseli / mL.Phambi kokutofa, phinda umise iiseli kwi-PBS kwaye uzihlalutye usebenzisa i-0.4% ye-trypan eluhlaza okwesibhakabhaka yokukhupha i-assay (iiseli eziphilayo> 90%).I-injection engaphantsi kwe-subcutaneous, i-1 × 107 Eca109 iiseli ze-Luc ezivela kwi-200 μ LPBS zafakwa kwisisu esisekhohlo sempuku nganye usebenzisa inaliti ye-27G.Emva kweveki enye yenaliti yeseli yethumba, i-Boforside I (25mg/kg ngempuku nganye) yatofwa kwisilonda kanye ngemini, ngelixa iimpuku ezili-10 ezisetyenziselwa unyango lwe-vector zanikwa umthamo olinganayo we-PBS [3].